Lisa M. Casanova, Soyoung Jeon, William A. Rutala, David J. Weber, Mark D. Sobsey
This study was conducted to investigate the effects of air temperature and humidity on coronaviruses. The two viruses examined are comparable to the SARS-CoV. Due to the great challenges in researching this virus, “replacement” viruses were chosen to gain a better insight into the survival and persistence of coronaviruses. This makes it possible to investigate the potential risk of transmission and virus precautions, such as SARS-CoV.
This study shows that viruses become inactive more quickly at 20 ° C than at 4 ° C, but the study showed in particular that the viruses have a greater chance of survival at low relative humidity. The infectious virus deposited on stainless steel surfaces persisted for at least 3 days at the correct humidity (50% RH, 20 ° C) and up to 28 days at the incorrect humidity (20%). Read more about the details of the study below.
Thin stainless steel test samples were inoculated with a liquid with a known number of viruses. The fluid used corresponds to the human body fluid (human secretions). The test samples were sealed in containers. After this 9 temperature and humidity controlled environments were created with the temperatures 4 ° C, 20 ° C and 40 ° C and humidity of 20% RH, 50% RH and 80% RH.
The rate of virus inactivation at the different controlled conditions was measured by viral plaque assays (number of viruses inducing a cytopathic effect) at time t (Nt), compared to the initial virus concentration (NO) expressed as log10. Sampling took place at different intervals for the different conditions.
The study suggests that the animal coronaviruses (TGEV and MHV) investigated could serve as conservative surrogates for modeling exposure, transmission risk, and control measures for human pathogenic viruses, such as SARS-CoV and other coronaviruses.